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Essay / Research Paper Abstract
This 4 page paper looks at hemoglobinopathies and hemoglobin electrophoresis(also known as Hgb electrophoresis) . The paper starts by explaining the basis of electrophoresis; what it is and how it works. The paper then considers the processes of hemoglobin electrophoresis and the meaning of different results and disorders that the test can identify. The bibliography cites 3 sources.
Page Count:
4 pages (~225 words per page)
File: TS14_TEhemoglobin.rtf
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Unformatted sample text from the term paper:
separating large molecules when they are in a mistier of other, alike molecules, for example DNA or proteins (Cahill, 1995). The process involved passing a current though a medium that
will contain the molecules. The basis is that when the current is passed though the medium this will cause the molecules to move. Positively charged molecules will travel to the
cathode and negatively charged molecules travelling to the anode. The molecules will travel art a different rate dependent on characteristics such as their size and their electrical charge, separation of
the molecules takes place using these differences. Mediums that are often used include agarose and acrylamide gels when nucleic acids and proteins are being searched for (Cahill, 1995).
Hemoglobin electrophoresis is used to detect a range of blood conditions but when the test is undertaken there is no need for any special preparation
of the blood or of the blood sample, however care should be taken where the subject has had a blood transfusion with the previous twelve weeks as this can skew
the results (Pagana and Deska, 1998). The blood is usually taken from the back of the back of the hand or the elbow, with the help of an elasticised band
placed around the upper arm in order to restrict blood supply and make collection easier (Pagana and Deska, 1998). The test will then
use the electrophoresis to identify the migrating molecules which move in bands, as seen with the description of electrophoresis above. The medium used to separate these molecules are cellulose acetate
at pH 8.6 and citrate agar at pH 6.2 as well as the use of Isoelectric focusing (Cahill, 1995). The blood samples will be electrophoresed with the use of
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