Sample Essay on:
Heart Development In Vertebrates, Chicks and Zebrafish

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Essay / Research Paper Abstract

8 pages in length. The writer technically discusses heart development in chicks, zebrafish and vertebrates. Bibliography lists 7 sources.

Page Count:

8 pages (~225 words per page)

File: LM1_TLCheartdev.rtf

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Unformatted sample text from the term paper:

Underlying the aspect of chick heart development, molecular components - while focal to the overall process - are still not complete and without speculation when it comes to human comprehension. Studies have been conducted as a means by which to bridge the misunderstood processes with the reality of what actually occurs, leading researchers to uncover the specific genetic composition associated with chick heart development (Sneesby, 2003). The author identified two gene fragments upon conducting differential display research which were found to be differentially expressed in the heart. Citing the isolation of full-length cDNA sequence was identified for NADH cytochrom b5 reductase as well as eukaryotic translation initiation factor-2b (eIF-2b), the author further discovered the expectation of gene upreglation during heart development, inasmuch as the heart "is the first functional organ to form in vertebrates and protein synthesis and cell metabolism at this stage of development is maximal" (Sneesby, 2003). Moreover, optimization and development of a subtractive hybridization method for use with small quantities of tissue or cells was found to be limited in the differential display approach. Subtractive hybridization was utilized as a means by which to concentrate on cardiac gene expression throughout the first developmental phases, which was accomplished in 9 embryos through the heart primordial and in stage 4 embryos Hamburger & Hamilton cardiogenic lateral plate mesoderm. Findings indicate how fifty-nine of the eighty-seven identified independent clones "matched known sequences with high homology, 25 matched unknown expressed sequence tag (EST) sequences with high homology, and 3 did not match any known sequence on the database. Known genes isolated included those involved in transcription, translation, cell signalling, RNA processing, and energy production" (Sneesby, 2003). Tagged for further research were an unknown gene named C1-20C and the high mobility group phosphoprotein A2 (HMGA2). ...

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